Publications

Selected publications

Malinverni D, Zamuner S, Barducci A, Nillegoda NB‡ , Rios PDL‡. Data-driven large-scale genomic analysis reveals an intricate phylogenetic and functional landscape in J-domain proteins. Proceedings of the National Academy of Sciences U S A. 2023 Aug 8;120(32):e2218217120. 

Zhang R, Malinverni D, Cyr DM, Rios PLD, Nillegoda NB*. J-domain protein chaperone circuits in proteostasis and disease. Trends in Cell Biology. 2023 Jan;33(1):30-47.

Wentink AS, Nillegoda NB, Feufel J, Ubartaitė G, Schneider CP, Rios PDL, Hennig J, Barducci A, Bukau B. Molecular dissection of amyloid disaggregation by human HSP70. Nature. 2020 Nov;587(7834):483-488.

Faust O, Abayev-Avraham M, Wentink AS, Maurer M, Nillegoda NB, London N, Bukau B, Rosenzweig R.

HSP40 proteins use class-specific regulation to drive HSP70 functional diversity. Nature. 2020 Nov;587(7834):489–494.

Nillegoda NB‡, Kirstein J, Szlachcic A, Berynskyy M, Stank A, Stengel F, Arnsburg K, Gao X, Scior A, Aebersold R, Guilbride DL, Wade RC, Morimoto RI, Mayer MP, Bukau B‡. Crucial HSP70 co-chaperone complex unlocks metazoan protein disaggregation. Nature. 2015 Aug 13;524(7564):247-51.

Kirstein J, Arnsburg K, Scior A, Szlachcic A, Guilbride DL, Morimoto RI, Bukau B‡, Nillegoda NB‡. In vivo properties of the disaggregase function of J-proteins and Hsc70 in Caenorhabditis elegans stress and aging. Aging Cell. 2017 Dec;16(6):1414-1424.

Rosenzweig R#, Nillegoda NB#, Mayer MP#, Bukau B#. The Hsp70 chaperone network. Nature Reviews Molecular Cell Biology. 2019 Nov;20(11):665-680.

(* indicates corresponding author, ‡ indicates co-corresponding author, # indicates equal contribution)

For a full list

Alberts-Visscher N, De Los Rios P, Kampinga HH, Nillegoda NB. Clearing the protein clutter in human cells: the role of protein disaggregases. Transactions of the Royal Society of South Africa. 2025. https://doi.org/10.1080/0035919X.2025.2515392 (In press)

Marszalek J, De Los Rios P, Cyr D, Mayer MP, Adupa V, Andréasson C, Blatch GL, Braun JEA, Brodsky JL, Bukau B, Chapple JP, Conz C, Dementin S, Genevaux P, Genest O, Goloubinoff P, Gestwicki J, Hammond CM, Hines JK, Ishikawa K, Joachimiak LA, Kirstein J, Liberek K, Mokranjac D, Nillegoda N, Ramos CHI, Rebeaud M, Ron D, Rospert S, Sahi C, Shalgi R, Tomiczek B, Ushioda R, Ustyantseva E, Ye Y, Zylicz M, Kampinga HH. J-domain proteins: From molecular mechanisms to diseases. Cell Stress Chaperones. 2024 Feb;29(1):21-33. doi: 10.1016/j.cstres.2023.12.002.

Mathangasinghe Y, Alberts N, Rosado C, Cox D, Payne N, Ormsby AR, Sakson R, Uthishtran S, Arumugam S, Hatters DM, Kampinga HH, Nillegoda NB. Replicative aging impedes stress-induced assembly of a key human protein disaggregase. bioRxiv (2022) doi:10.1101/2022.06.25.497591.

Malinverni D, Zamuner S, Rebeaud ME, Barducci A, Nillegoda NB, De Los Rios P. Data-driven large-scale genomic analysis reveals an intricate phylogenetic and functional landscape in J-domain proteins. Proc Natl Acad Sci U S A. 2023 Aug 8;120(32):e2218217120. doi: 10.1073/pnas.2218217120.

van Oosten-Hawle P, Backe SJ, Ben-Zvi A, Bourboulia D, Brancaccio M, Brodsky J, Clark M, Colombo G, Cox MB, De Los Rios P, Echtenkamp F, Edkins A, Freeman B, Goloubinoff P, Houry W, Johnson J, LaPointe P, Li W, Mezger V, Neckers L, Nillegoda NB, et al. Second Virtual International Symposium on Cellular and Organismal Stress Responses, September 8-9, 2022. Cell Stress Chaperones. 2023 Jan;28(1):1-9. doi: 10.1007/s12192-022-01318-5.

Zhang R, Malinverni D, Cyr DM, Rios PL, Nillegoda NB. J-domain protein chaperone circuits in proteostasis and disease. Trends Cell Biol. 2023 Jan;33(1):30-47. doi: 10.1016/j.tcb.2022.05.004.

Cox D, Ang CS, Nillegoda NB, Reid GE, Hatters DM. Hidden information on protein function in censuses of proteome foldedness. Nat Commun. 2022 Apr 14;13(1):1992. doi: 10.1038/s41467-022-29661-2.

Mathangasinghe Y, Fauvet B, Jane SM, Goloubinoff P, Nillegoda NB. The Hsp70 chaperone system: distinct roles in erythrocyte formation and maintenance. Haematologica. 2021 Jun 1;106(6):1519-1534. doi: 10.3324/haematol.2019.233056.

Wentink AS, Nillegoda NB, Feufel J, Ubartaitė G, Schneider CP, De Los Rios P, Hennig J, Barducci A, Bukau B. Author Correction: Molecular dissection of amyloid disaggregation by human HSP70. Nature. 2021 Jan;589(7841):E2. doi: 10.1038/s41586-020-03090-x.

Faust O, Abayev-Avraham M, Wentink AS, Maurer M, Nillegoda NB, London N, Bukau B, Rosenzweig R. HSP40 proteins use class-specific regulation to drive HSP70 functional diversity. Nature. 2020 Nov;587(7834):489-494. doi: 10.1038/s41586-020-2906-4.

Wentink AS, Nillegoda NB, Feufel J, Ubartaitė G, Schneider CP, De Los Rios P, Hennig J, Barducci A, Bukau B. Molecular dissection of amyloid disaggregation by human HSP70. Nature. 2020 Nov;587(7834):483-488. doi: 10.1038/s41586-020-2904-6.

Serlidaki D, van Waarde MAWH, Rohland L, Wentink AS, Dekker SL, Kamphuis MJ, Boertien JM, Brunsting JF, Nillegoda NB, et al. Functional diversity between HSP70 paralogs caused by variable interactions with specific co-chaperones. J Biol Chem. 2020 May 22;295(21):7301-7316. doi: 10.1074/jbc.RA119.012449.

Alberts N, Mathangasinghe Y, Nillegoda NB. In Situ Monitoring of Transiently Formed Molecular Chaperone Assemblies in Bacteria, Yeast, and Human Cells. J Vis Exp. 2019 Sep 2;(151). doi: 10.3791/60172.

Rosenzweig R, Nillegoda NB, Mayer MP, Bukau B. The Hsp70 chaperone network. Nat Rev Mol Cell Biol. 2019 Nov;20(11):665-680. doi: 10.1038/s41580-019-0133-3.

Kampinga HH, Andreasson C, Barducci A, Cheetham ME, Cyr D, Emanuelsson C, Genevaux P, Gestwicki JE, Goloubinoff P, et al. Function, evolution, and structure of J-domain proteins. Cell Stress Chaperones. 2019 Jan;24(1):7-15. doi: 10.1007/s12192-018-0948-4.

Nillegoda NB, Wentink AS, Bukau B. Protein Disaggregation in Multicellular Organisms. Trends Biochem Sci. 2018 Apr;43(4):285-300. doi: 10.1016/j.tibs.2018.02.003.

Kirstein J, Arnsburg K, Scior A, Szlachcic A, Guilbride DL, Morimoto RI, Bukau B, Nillegoda NB. In vivo properties of the disaggregase function of J-proteins and Hsc70 in Caenorhabditis elegans stress and aging. Aging Cell. 2017 Dec;16(6):1414-1424. doi: 10.1111/acel.12686.

Garcia VM, Nillegoda NB, Bukau B, Morano KA. Substrate binding by the yeast Hsp110 nucleotide exchange factor and molecular chaperone Sse1 is not obligate for its biological activities. Mol Biol Cell. 2017 Jul 15;28(15):2066-2075. doi: 10.1091/mbc.E17-01-0070.

Nillegoda NB, Stank A, Malinverni D, Alberts N, Szlachcic A, Barducci A, De Los Rios P, Wade RC, Bukau B. Evolution of an intricate J-protein network driving protein disaggregation in eukaryotes. Elife. 2017 May 15;6:e24560. doi: 10.7554/eLife.24560.

Żwirowski S, Kłosowska A, Obuchowski I, Nillegoda NB, Piróg A, Ziętkiewicz S, Bukau B, Mogk A, Liberek K. Hsp70 displaces small heat shock proteins from aggregates to initiate protein refolding. EMBO J. 2017 Mar 15;36(6):783-796. doi: 10.15252/embj.201593378.

Nillegoda NB, Bukau B. Metazoan Hsp70-based protein disaggregases: emergence and mechanisms. Front Mol Biosci. 2015 Oct 9;2:57. doi: 10.3389/fmolb.2015.00057.

Gao X, Carroni M, Nussbaum-Krammer C, Mogk A, Nillegoda NB, Szlachcic A, Guilbride DL, Saibil HR, Mayer MP, Bukau B. Human Hsp70 Disaggregase Reverses Parkinson's-Linked α-Synuclein Amyloid Fibrils. Mol Cell. 2015 Sep 3;59(5):781-93. doi: 10.1016/j.molcel.2015.07.012.

Nillegoda NB, Kirstein J, Szlachcic A, Berynskyy M, Stank A, Stengel F, Arnsburg K, Gao X, Scior A, et al. Crucial HSP70 co-chaperone complex unlocks metazoan protein disaggregation. Nature. 2015 Aug 13;524(7564):247-51. doi: 10.1038/nature14884.

Hsieh TY, Nillegoda NB, Tyedmers J, Bukau B, Mogk A, Kramer G. Monitoring protein misfolding by site-specific labeling of proteins in vivo. PLoS One. 2014 Jun 10;9(6):e99395. doi: 10.1371/journal.pone.0099395.

Rampelt H, Kirstein-Miles J, Nillegoda NB, Chi K, Scholz SR, Morimoto RI, Bukau B. Metazoan Hsp70 machines use Hsp110 to power protein disaggregation. EMBO J. 2012 Nov 5;31(21):4221-35. doi: 10.1038/emboj.2012.264.

Theodoraki MA, Nillegoda NB, Saini J, Caplan AJ. A network of ubiquitin ligases is important for the dynamics of misfolded protein aggregates in yeast. J Biol Chem. 2012 Jul 6;287(28):23911-22. doi: 10.1074/jbc.M112.341164.

Mandal AK, Theodoraki MA, Nillegoda NB, Caplan AJ. Role of molecular chaperones in biogenesis of the protein kinome. Methods Mol Biol. 2011;787:75-81. doi: 10.1007/978-1-61779-295-3_6.

Nillegoda NB, Theodoraki MA, Mandal AK, Mayo KJ, Ren HY, Sultana R, Wu K, Johnson J, Cyr DM, Caplan AJ. Ubr1 and Ubr2 function in a quality control pathway for degradation of unfolded cytosolic proteins. Mol Biol Cell. 2010 Jul 1;21(13):2102-16. doi: 10.1091/mbc.e10-02-0098.

Mandal AK, Gibney PA, Nillegoda NB, Theodoraki MA, Caplan AJ, Morano KA. Hsp110 chaperones control client fate determination in the hsp70-Hsp90 chaperone system. Mol Biol Cell. 2010 May 1;21(9):1439-48. doi: 10.1091/mbc.e09-09-0779.

Mandal AK, Nillegoda NB, Chen JA, Caplan AJ. Ydj1 protects nascent protein kinases from degradation and controls the rate of their maturation. Mol Cell Biol. 2008 Jul;28(13):4434-44. doi: 10.1128/MCB.00543-08.

Jones J, Wu K, Yang Y, Guerrero C, Nillegoda N, Pan ZQ, Huang L. A targeted proteomic analysis of the ubiquitin-like modifier nedd8 and associated proteins. J Proteome Res. 2008 Mar;7(3):1274-87. doi: 10.1021/pr700749v.

Mandal AK, Lee P, Chen JA, Nillegoda N, Heller A, DiStasio S, Oen H, Victor J, Nair DM, Brodsky JL, Caplan AJ. Cdc37 has distinct roles in protein kinase quality control that protect nascent chains from degradation and promote posttranslational maturation. J Cell Biol. 2007 Jan 29;176(3):319-28. doi: 10.1083/jcb.200604106.